Cells were blocked with 2 bovine serum albumin phosphate buffered saline BSA PBS, incubated first with primary antibodies 1 500 dilution, and then with Alexa Fluor 594 conjugated secondary antibodies 1 1000 dilution, followed by staining with BODIPY 493 503 and DAPI ivermectina stromectol precio Antianginal Amiodarone see above and 2 Thiouracil